anti hes1 rabbit antibody Search Results


rabbit anti hes 1  (Boster Bio)
90
Boster Bio rabbit anti hes 1
Rabbit Anti Hes 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti hes 1/product/Boster Bio
Average 90 stars, based on 1 article reviews
rabbit anti hes 1 - by Bioz Stars, 2026-03
90/100 stars
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hes1  (Merck KGaA)
90
Merck KGaA hes1
mRNA expression of NOTCH1-4 receptors, JAGGED1 ligand and <t>HES1</t> target gene were determined by qRT-PCR. Gene levels normalized to the housekeeping gene Gadph are shown as percentage of change of NF average (which was considered 100%) (NF = non functioning adenoma; ACTH = corticotropinoma; GH = somatotropinoma).
Hes1, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hes1/product/Merck KGaA
Average 90 stars, based on 1 article reviews
hes1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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mRNA expression of NOTCH1-4 receptors, JAGGED1 ligand and HES1 target gene were determined by qRT-PCR. Gene levels normalized to the housekeeping gene Gadph are shown as percentage of change of NF average (which was considered 100%) (NF = non functioning adenoma; ACTH = corticotropinoma; GH = somatotropinoma).

Journal: Oncotarget

Article Title: Notch system is differentially expressed and activated in pituitary adenomas of distinct histotype, tumor cell lines and normal pituitaries

doi: 10.18632/oncotarget.19046

Figure Lengend Snippet: mRNA expression of NOTCH1-4 receptors, JAGGED1 ligand and HES1 target gene were determined by qRT-PCR. Gene levels normalized to the housekeeping gene Gadph are shown as percentage of change of NF average (which was considered 100%) (NF = non functioning adenoma; ACTH = corticotropinoma; GH = somatotropinoma).

Article Snippet: For mouse tissue the following antibodies were used: Rabbit polyclonal antibody against NOTCH1 and NOTCH2 (dilution 1:700, Merck Millipore), NOTCH3 (dilution 1:200, Santa Cruz Biotechnology Inc), HES1 (dilution 1:400, Merck Millipore).

Techniques: Expressing, Quantitative RT-PCR

Relation between mRNA levels of NOTCH1-4 and JAGGED1 ( n = 13, 8, 13, 6 ( A – D )) or HES1 ( n = 11, 10, 11, 12 ( E – H )) was determined in all adenomas tested. The equation of linear regression, R2 coefficient of determination and coefficient of Spearman are shown in each graph. p ≤ 0.05 denotes a significant correlation.

Journal: Oncotarget

Article Title: Notch system is differentially expressed and activated in pituitary adenomas of distinct histotype, tumor cell lines and normal pituitaries

doi: 10.18632/oncotarget.19046

Figure Lengend Snippet: Relation between mRNA levels of NOTCH1-4 and JAGGED1 ( n = 13, 8, 13, 6 ( A – D )) or HES1 ( n = 11, 10, 11, 12 ( E – H )) was determined in all adenomas tested. The equation of linear regression, R2 coefficient of determination and coefficient of Spearman are shown in each graph. p ≤ 0.05 denotes a significant correlation.

Article Snippet: For mouse tissue the following antibodies were used: Rabbit polyclonal antibody against NOTCH1 and NOTCH2 (dilution 1:700, Merck Millipore), NOTCH3 (dilution 1:200, Santa Cruz Biotechnology Inc), HES1 (dilution 1:400, Merck Millipore).

Techniques:

( A – C ) Gene expression normalized to Gapdh in AtT20 cells and mouse pituitaries determined by qRT-PCR and expressed as percentage of change of the normal pituitaries which were considered 100%. *p = 0.0003 for Notch3, Notch1,2,4 NS, n = 3–5; 3–8 for AtT20 and pituitary respectively (A), p = 0.0111 and p = 0.0105 for the Notch ligands Dll1 and Jagged1 , respectively vs pituitary and (B) p = 0.0016, 0.0184 and 0.0032 for the Notch target genes Hes1, Hes5 and Hey2 , respectively, vs. pituitary n = 2–3; 3 for AtT20 and pituitary respectively (C). ( D , E ) Active and membrane domains of NOTCH1-3 receptor levels determined by Western Blot: Bars show the mean of the receptor normalized to β actin levels, expressed as the percentage of normal mouse pituitary. *p = 0.026 for NOTCH1, p < 0.0001 for NOTCH2 and p = 0.0146 for NOTCH3 vs. pituitary, for the active domains (80KDa) n = 3–6; 5–7 for AtT20 and pituitary respectively (D). No significant differences were found for the membrane domain (110 KDa) n = 3–6; 3–7 for AtT20 and pituitary respectively (E). Representative Western blots showing the active and membrane domains of NOTCH receptors in normal pituitaries (Pit) and ATt20 cells ( F ).

Journal: Oncotarget

Article Title: Notch system is differentially expressed and activated in pituitary adenomas of distinct histotype, tumor cell lines and normal pituitaries

doi: 10.18632/oncotarget.19046

Figure Lengend Snippet: ( A – C ) Gene expression normalized to Gapdh in AtT20 cells and mouse pituitaries determined by qRT-PCR and expressed as percentage of change of the normal pituitaries which were considered 100%. *p = 0.0003 for Notch3, Notch1,2,4 NS, n = 3–5; 3–8 for AtT20 and pituitary respectively (A), p = 0.0111 and p = 0.0105 for the Notch ligands Dll1 and Jagged1 , respectively vs pituitary and (B) p = 0.0016, 0.0184 and 0.0032 for the Notch target genes Hes1, Hes5 and Hey2 , respectively, vs. pituitary n = 2–3; 3 for AtT20 and pituitary respectively (C). ( D , E ) Active and membrane domains of NOTCH1-3 receptor levels determined by Western Blot: Bars show the mean of the receptor normalized to β actin levels, expressed as the percentage of normal mouse pituitary. *p = 0.026 for NOTCH1, p < 0.0001 for NOTCH2 and p = 0.0146 for NOTCH3 vs. pituitary, for the active domains (80KDa) n = 3–6; 5–7 for AtT20 and pituitary respectively (D). No significant differences were found for the membrane domain (110 KDa) n = 3–6; 3–7 for AtT20 and pituitary respectively (E). Representative Western blots showing the active and membrane domains of NOTCH receptors in normal pituitaries (Pit) and ATt20 cells ( F ).

Article Snippet: For mouse tissue the following antibodies were used: Rabbit polyclonal antibody against NOTCH1 and NOTCH2 (dilution 1:700, Merck Millipore), NOTCH3 (dilution 1:200, Santa Cruz Biotechnology Inc), HES1 (dilution 1:400, Merck Millipore).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

( A – C ) Differential gene expression normalized to Gapdh and expressed as percentage of change of the normal pituitaries considered 100% determined by qRT-PCR. For the figures in this panel, different letters indicate significant differences ( p ≤ 0.05) between groups. (A) Notch1-4 receptors n = 4–5, 5, 4, 4–6 for MMQ, GH3, GH3 in vivo tumors and rat pituitaries. (B) Notch ligands Dll1 and Jagged1 n = 3, 3, 3, 2 and (C) Notch target genes Hes1, Hey1 and Hey2 n = 3, 3, 3, 2 for MMQ, GH3, GH3 in vivo tumors and rat pituitaries, respectively. ( D – E ) Active and membrane domains of NOTCH1-3 receptors were determined by Western blot. Bars show the mean of the relation of the receptor levels to β actin expression, showed as the percentage of normal rat pituitary. (D) The active domains (80KDa): n = 5–6; 5, 3, 6. (E) The membrane domains (110KDa): n = 3–6, 2–5, 0–3, 3–6 for MMQ, GH3, GH3 tumors and normal rat pituitaries. ( F ) Representative Western blots showing the active and membrane domains of NOTCH receptors in MMQ, GH3, GH3 in vivo tumors and normal pituitaries.

Journal: Oncotarget

Article Title: Notch system is differentially expressed and activated in pituitary adenomas of distinct histotype, tumor cell lines and normal pituitaries

doi: 10.18632/oncotarget.19046

Figure Lengend Snippet: ( A – C ) Differential gene expression normalized to Gapdh and expressed as percentage of change of the normal pituitaries considered 100% determined by qRT-PCR. For the figures in this panel, different letters indicate significant differences ( p ≤ 0.05) between groups. (A) Notch1-4 receptors n = 4–5, 5, 4, 4–6 for MMQ, GH3, GH3 in vivo tumors and rat pituitaries. (B) Notch ligands Dll1 and Jagged1 n = 3, 3, 3, 2 and (C) Notch target genes Hes1, Hey1 and Hey2 n = 3, 3, 3, 2 for MMQ, GH3, GH3 in vivo tumors and rat pituitaries, respectively. ( D – E ) Active and membrane domains of NOTCH1-3 receptors were determined by Western blot. Bars show the mean of the relation of the receptor levels to β actin expression, showed as the percentage of normal rat pituitary. (D) The active domains (80KDa): n = 5–6; 5, 3, 6. (E) The membrane domains (110KDa): n = 3–6, 2–5, 0–3, 3–6 for MMQ, GH3, GH3 tumors and normal rat pituitaries. ( F ) Representative Western blots showing the active and membrane domains of NOTCH receptors in MMQ, GH3, GH3 in vivo tumors and normal pituitaries.

Article Snippet: For mouse tissue the following antibodies were used: Rabbit polyclonal antibody against NOTCH1 and NOTCH2 (dilution 1:700, Merck Millipore), NOTCH3 (dilution 1:200, Santa Cruz Biotechnology Inc), HES1 (dilution 1:400, Merck Millipore).

Techniques: Expressing, Quantitative RT-PCR, In Vivo, Western Blot

( A – C ) Gene expression determined by qRT-PCR in pituitaries of lacDrd2KO and Drd2l oxP/loxP counterparts normalized to Gapdh and expressed as percentage of change of the Drd2 loxP/loxP pituitaries considered 100%. Bars show mean values. Notch1 *p = 0.0431, Notch3 *p = 0.0089 vs. Drd2 loxp/loxp ; Notch2, 4 NS, n = 7, 5 for lacDrd2KO and Drd2 loxP/loxP mice, respectively (A). Notch ligands Dll1 and Jagged1 NS (B), and Notch target genes Hes1 (* p = 0.0106 lacDrd2KO vs. Drd2 loxP/loxP ) , Hes5 and Hey2 , NS (C); n = 7, 5 for lacDrd2KO and Drd2 loxP/loxP mice, respectively. ( D – E ) The expression of the active and membrane domains of NOTCH1-3 receptors was determined by Western blot, related to the correspondent β actin levels, and expressed as the percentage of Drd2 loxP/loxP pituitary average. *p = 0.01 for NOTCH1, NOTCH2, 3 NS active domain, p =0.0604 and p = 0.0042 for NOTCH2 and NOTCH3 membrane domains, respectively for lacDrd2KO vs Drd2 loxP/loxP m ice. n = 4, 3–4 for lacDrd2KO and Drd2 loxP/loxP . Representative Western blots showing the active and membrane domains of NOTCH receptors in lacDrd2KO and Drd2 loxP/loxP ( F ). Representative microphotographies of NOTCH1-3 and HES1 Immunohistochemistry performed in lacDrd2KO and Drd2 loxP/loxP mice ( G ).

Journal: Oncotarget

Article Title: Notch system is differentially expressed and activated in pituitary adenomas of distinct histotype, tumor cell lines and normal pituitaries

doi: 10.18632/oncotarget.19046

Figure Lengend Snippet: ( A – C ) Gene expression determined by qRT-PCR in pituitaries of lacDrd2KO and Drd2l oxP/loxP counterparts normalized to Gapdh and expressed as percentage of change of the Drd2 loxP/loxP pituitaries considered 100%. Bars show mean values. Notch1 *p = 0.0431, Notch3 *p = 0.0089 vs. Drd2 loxp/loxp ; Notch2, 4 NS, n = 7, 5 for lacDrd2KO and Drd2 loxP/loxP mice, respectively (A). Notch ligands Dll1 and Jagged1 NS (B), and Notch target genes Hes1 (* p = 0.0106 lacDrd2KO vs. Drd2 loxP/loxP ) , Hes5 and Hey2 , NS (C); n = 7, 5 for lacDrd2KO and Drd2 loxP/loxP mice, respectively. ( D – E ) The expression of the active and membrane domains of NOTCH1-3 receptors was determined by Western blot, related to the correspondent β actin levels, and expressed as the percentage of Drd2 loxP/loxP pituitary average. *p = 0.01 for NOTCH1, NOTCH2, 3 NS active domain, p =0.0604 and p = 0.0042 for NOTCH2 and NOTCH3 membrane domains, respectively for lacDrd2KO vs Drd2 loxP/loxP m ice. n = 4, 3–4 for lacDrd2KO and Drd2 loxP/loxP . Representative Western blots showing the active and membrane domains of NOTCH receptors in lacDrd2KO and Drd2 loxP/loxP ( F ). Representative microphotographies of NOTCH1-3 and HES1 Immunohistochemistry performed in lacDrd2KO and Drd2 loxP/loxP mice ( G ).

Article Snippet: For mouse tissue the following antibodies were used: Rabbit polyclonal antibody against NOTCH1 and NOTCH2 (dilution 1:700, Merck Millipore), NOTCH3 (dilution 1:200, Santa Cruz Biotechnology Inc), HES1 (dilution 1:400, Merck Millipore).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunohistochemistry